SP Sepharose FF has “smooth” pores ligands are covalently attached without using grafting techniques. In this doctoral thesis the cation exchange resins SP Sepharose FF, Capto S, Capto S ImpAct and S HyperD will be compared. The impact of the type of mass transport on the adsorption and desorption rate or competitive binding effects of the two-component mixtures on the respective media will be investigated using established experimental techniques The intraparticle mass transport will be evaluated using resins with four distinct types of solid phase characteristics inside the pore space. The core of the PhD thesis will be the investigation of adsorption and desorption processes of two-component systems comprising model proteins of different properties and sizes. Recent studies on two- or multi-component mixtures and their adsorption properties based on one or the other mechanism have mostly been restricted to protein variants or only one type of resin. The dominant driving force depends on the composition of the adsorbent, the properties of the respective proteins as well as the operating conditions, e.g.
There is a variety of different resins with diverse stationary phase characteristics available which directly influence the mass transport of the protein species. Intraparticle mass transport is based on pore diffusion, solid-phase diffusion, or a combination of both. The protein adsorption rate is usually controlled by diffusion inside the particles.
Designing a protein chromatography process requires an understanding of the rate at with which proteins bind to the chromatography media.
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